The aim of this experiment was to use Bradford

The aim of this experiment was to use Bradford protein assay in order to determine the
concentration of protein in a ground beef tissue sample using ground beef extract and a
potassium phosphate buffer. The total protein concentration contained in the ground beef
sample was discovered to be 36.57 mg/g, as shown on table 1.1. Two of the 1 in 20 dilutions
were found to be in the range of the protein standard curve as shown on table 1.1. An R^2
value of 0.9996 was calculated through the standard curve graph, showing a very comparable
linear fit as displayed on Figure 1.1. This confirmed the hypothesis that a high concentration of
protein would be found in the ground beef sample. Ground beef comes from the skeletal
muscle of a cow and therefore contains tissues and fat. The tissues found in this skeletal muscle
are highly soluble proteins, which allows protein concentrations to be accurately measured in
specific conditions (Doherty et al., 2004). When comparing protein concretions in ground beef
found to literature values of previous studies who also used the Bradford protein assay method,
it was discovered that the semimembranosus muscle beef tissue had a protein concentration of
44.6 mg/g (Claus and Sørheim, 2006). This value shows a 19.8% difference from the value
obtained through experimentation. Experimental and literature values may show this large
difference, due to the method in which the ground beef extract was obtained. For example,
stratified muscle cells contain a high number of mitochondria within its form. Mitochondria
contain membranes and therefore consist of protein (Mootha et al., 2003). During the
extraction process, it is probable that a low number of mitochondria were included in the
sample and therefore, the amount of protein found in this sample would be of a lower
concentration. Although, protein concentrations in the experimental and literature values
differed, this experiment showed successful results by the Bradford protein assay method and
helped confirm the hypothesis that a measurable amount of protein would be discovered
within the ground beef sample. 

The aim of this experiment was to use Bradford protein assay in order to determine the
concentration of protein in a ground beef tissue sample using ground beef extract and a
potassium phosphate buffer. The total protein concentration contained in the ground beef
sample was discovered to be 36.57 mg/g, as shown on table 1.1. Two of the 1 in 20 dilutions
were found to be in the range of the protein standard curve as shown on table 1.1. An R^2
value of 0.9996 was calculated through the standard curve graph, showing a very comparable
linear fit as displayed on Figure 1.1. This confirmed the hypothesis that a high concentration of
protein would be found in the ground beef sample. Ground beef comes from the skeletal
muscle of a cow and therefore contains tissues and fat. The tissues found in this skeletal muscle
are highly soluble proteins, which allows protein concentrations to be accurately measured in
specific conditions (Doherty et al., 2004). When comparing protein concretions in ground beef
found to literature values of previous studies who also used the Bradford protein assay method,
it was discovered that the semimembranosus muscle beef tissue had a protein concentration of
44.6 mg/g (Claus and Sørheim, 2006). This value shows a 19.8% difference from the value
obtained through experimentation. Experimental and literature values may show this large
difference, due to the method in which the ground beef extract was obtained. For example,
stratified muscle cells contain a high number of mitochondria within its form. Mitochondria
contain membranes and therefore consist of protein (Mootha et al., 2003). During the
extraction process, it is probable that a low number of mitochondria were included in the
sample and therefore, the amount of protein found in this sample would be of a lower
concentration. Although, protein concentrations in the experimental and literature values
differed, this experiment showed successful results by the Bradford protein assay method and
helped confirm the hypothesis that a measurable amount of protein would be discovered
within the ground beef sample. 

x

Hi!
I'm Elaine!

Would you like to get a custom essay? How about receiving a customized one?

Check it out