techniqueIntroduction Biochemical techniques known to methods set

techniqueIntroduction               Biochemical techniques known to methods set .  That are able to check enzyme activity that is assay procedure scientist to analyze the substance that present on living organisms  and also underlying the process of life chemical reaction . The most important technique are used in such as testing for that are not legal drug that abuse in athletic and also observed blood glucose level in diabetic.To perform the analysis of living molecules in of biological and also in system to design the strategy that identify molecules in living system .  That separate original form of hundreds of molecules that found at molecular level that are present in extract from living sample and observe its function  .  Quantitative sample or non-quantitative samples are involved in test of biochemical. Number of molecules is important. Enzyme activity are simplest category of enzyme assay procedure that are help in measure in spectrophotometry and also gel staining to identify the quality of protein   & concentration.Centrifugation The available of method for used as purified of biomolecules that are range in simple precipitation, centrifugation .Impose process of centrifugation in which fast spinning high speed of centrifugal forces on molecules of living organisms. That are based on weight of different type of separation.Electrophoresis This technique is useful for charges of molecules of living organisms and size of molecules of living organisms and charges of molecules in living orgainisms they are migrated adopted by anodes and cathode poles of charges in electric field Gel Electrophoresis This technique is useful for separation of molecules and studies of DNA  , protein and lipids in analysis of biomolecules technique .After sequencing is completed of DNA at human genome of different type is dependent on gel electrophoresis.Two Dimensional Gel Electrophoresis The two dimensional of gel electrophoresis of proteome that are started in several laboratories.Chromatography This type of technique are sensitive and effective in separating and mixture of molecules most common used in quantitative liquid or gaseous in solution method that are consist of mixture tests and they are flow through tube and solid material are purely divided and chemicals are active groups and in which coated on upper surface . mixture separate are different component by different rate they are travel through pourous stationary depending on interact column diffuse in small molecules . They will be delayed in matrix column are quickly in molecules that are larger.Exchange chromatographyUltra centrifugation and gel electrophoresis both are useful in determination of molecular weight in biomolecules separating of biomolecules in charge stationary material of chromatography column . this pathway is known as exchange chromatography. Cell fractionation Cellular component used to separate in biochemical techniques . Cellular location was demonstrated in this type of technique they are also useful in the purpose of protein purified , disease stage diagnosis at cellular level .Homogenization Typically used to homogeniesd tissue in solution of buffer to stop osmotic damaged . the grinding , mincing , chopping , presuure exchange are the mechanisms of homogenization grinding of cell presence of normal PH , ionic , temperature . Filtration   That is done by pouring through suction of filter.Purification That is done by different centrifugal forces .Spectrophotometry It is the measurement of properties and function of wavelength. they are deals with visible light , ultraviolet .It is the quantitative analysis of biomolecules in biochemical techniques . Spectrometer are photometry so they are known to spectrophotometry .It is use for measurement of solution of reflection.Application in biochmistryUsed in isolation of of proteinInvolved in RNA , DNA Involved in enzyme reactions


I'm Mary!

Would you like to get a custom essay? How about receiving a customized one?

Check it out