In Figure. 7a&b FT-IR analysis is used to identify and get an approximate idea of the possible biomolecules that are responsible for capping and stabilization of the Cu-NPs with the Trifolium resupinatum leaf extract. A strong peak at 3447 cm-1 can be attributed to O-H and N-H of phenol and amide groups, Figure. (7a). this peak shifted to lower field at 3422s cm-1 in the synthesized Cu-NPs, Figure. (7b). The bands at 2928 cm-1, 2850 cm-1 and 2924 cm-1, 2841 cm-1 in Figure. (7a) and Figure. (7b) are assigned to –CH2 and C-H stretching mode in alkanes. The amazing peak appears at 1540 cm-1 which attributed to C=C stretching of the aromatic ring. The small sharp peak at 1701 cm-1 in Trafilum leaf extract could be attributed to C=C stretching vibrations about C=O amide conjugated C=O of the proteins that are responsible for capping and stabilizing of Cu-NPs. The peaks observed in the range of 698-1449 cm-1 have been assigned to alcohols and phenolic groups, C-N stretching vibrations of aliphatic and aromatic amines. The major peak was observed to be 525 cm-1 should be a stretching of Cu-O. The bands at 1628 cm-1 of the leaf extract shifted to lower field at 1608 cm-1, in the product while the bands at 1449 cm-1 shifted to a higher field at 1469 cm-1 and the peak at 1322 cm-1 shifted to 1347 cm-1. These bands assigned to C-O stretching, C=O stretching and N-H bending. The shifting in these bands is clearly indicating that the coordination of carboxylic acids in the protein of Trafilum leaf extract with Cu-NPs play a major role on dispersing, stabilizing and capping of Cu-NPs 21.