For many infectious diseases, timely,accurate and rapid diagnosis coupled with prompt effective treatment mayimprove patient outcomes and reduce transmission through vulnerable populations1. Understanding the structure of DNA enables to improve the existing methodsor to develop new models for the diagnosis of genetic disease. Theidentification of microbial diseases or genetic disorders is a long process, takesapproximately 1-5 days 2; which has an important effect on morbidity ratio. Identificationprocess therefore must be as fast as possible. Furthermore, DNA detectiontechnology could be a solution for important bacterial transmitted disease. Becauseof the bacteria, Chlamydia trachomatis, which could be detected by antigendetection technologies; 3 to 4 million people are infected occur annually inthe United States and that 89 million cases occur worldwide 3.
Due to thefact that the detection technology could save lots of people’s lives, it mustbe affordable for lots of people. As aconsequence, more rapid, sensitive, affordable and specific technology shouldbe developed to take the place of slow and limited methods.The nucleic acid amplification testing (NAAT)technique is one of the most effective and advantageous method formicrobiological detection; which could be used for rapid on-site detection ofenvironmental, food-borne and water borne pathogens and point-of-care clinicaldiagnostics 4. Nucleic acid amplification testing (NAAT) techniques interestDNA sequences directly, allowing further clinical information to be detectedfrom either patient or pathogen; the aim of NAAT is to identify and potentiallyquantify specific nucleic acid sequences from clinical samples 2. NAAT technologyis recently developed as an appropriate way of point-to-care detection;however, the new technology is complex and too expensive for widespreaddeployment. The basic aim of the project is to develop NAAT process forpoint-of-care detection; which is affordable and simple to use.