Expression the translocation of mRNA and peptidyl-tRNA from

Expression Analysis of Dph genes 1-7 underheat stress in Arabidopsis thalianaIntroduction:Diphthamideis a modified histidine residue that is present in eurkaryotic and archealelongation factor 2(eEF2) as a result of post translation modification. Diphthamideis targeted by diphtheria toxin, produced by Corynebacterium diphtheriae and exotoxin A, produced by Pseudomonas aeruginosa that are involvedin catalyzing the transfer of ADP-ribose from NAD+ to eEF2. eEF2catalyzes the translocation of mRNA and peptidyl-tRNA from ribosome A site to Psite.

ThisADP ribosylation leads to inactivation of EF2, hence the protein synthesisceases and the cell dies (Honjo et al., 1968, Jorgensen et al., 2006).  Biosynthesis of Diphthamide:Biosynthesis ofDiphthamide involves stepwise additions to His715(His669in yeast) residue of eukaryotic elongation factor 2. Biosynthesis begins withtransfer of the 3-amino-3-carboxypropyl (ACP) group of S-adenosylmethionine(AdoMet)to the imidazole C-2 of the precursor histidine residue. Trimethylation of newlyformed amino group using AdoMet as a source of methyl group leads to theformation of Diphthine.

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Diphthine is converted to Diphthamide by ATP dependentamidation of carboxyl group. Mutants defective in Diphthamide biosynthesis havebeen isolated by selection for resistance to the actions of Diphtheria toxinand exotoxin A.These mutations are categorized into five complementation groups   Expression Analysis of Dph genes 1-7 underheat stress in Arabidopsis thalianaIntroduction:Diphthamideis a modified histidine residue that is present in eurkaryotic and archealelongation factor 2(eEF2) as a result of post translation modification. Diphthamideis targeted by diphtheria toxin, produced by Corynebacterium diphtheriae and exotoxin A, produced by Pseudomonas aeruginosa that are involvedin catalyzing the transfer of ADP-ribose from NAD+ to eEF2. eEF2catalyzes the translocation of mRNA and peptidyl-tRNA from ribosome A site to Psite.

ThisADP ribosylation leads to inactivation of EF2, hence the protein synthesisceases and the cell dies (Honjo et al., 1968, Jorgensen et al., 2006).

 Biosynthesis of Diphthamide:Biosynthesis ofDiphthamide involves stepwise additions to His715(His669in yeast) residue of eukaryotic elongation factor 2. Biosynthesis begins withtransfer of the 3-amino-3-carboxypropyl (ACP) group of S-adenosylmethionine(AdoMet)to the imidazole C-2 of the precursor histidine residue. Trimethylation of newlyformed amino group using AdoMet as a source of methyl group leads to theformation of Diphthine. Diphthine is converted to Diphthamide by ATP dependentamidation of carboxyl group. Mutants defective in Diphthamide biosynthesis havebeen isolated by selection for resistance to the actions of Diphtheria toxinand exotoxin A.These mutations are categorized into five complementation groups    Expression Analysis of Dph genes 1-7 underheat stress in Arabidopsis thalianaIntroduction:Diphthamideis a modified histidine residue that is present in eurkaryotic and archealelongation factor 2(eEF2) as a result of post translation modification.

Diphthamideis targeted by diphtheria toxin, produced by Corynebacterium diphtheriae and exotoxin A, produced by Pseudomonas aeruginosa that are involvedin catalyzing the transfer of ADP-ribose from NAD+ to eEF2. eEF2catalyzes the translocation of mRNA and peptidyl-tRNA from ribosome A site to Psite.ThisADP ribosylation leads to inactivation of EF2, hence the protein synthesisceases and the cell dies (Honjo et al., 1968, Jorgensen et al., 2006).  Biosynthesis of Diphthamide:Biosynthesis ofDiphthamide involves stepwise additions to His715(His669in yeast) residue of eukaryotic elongation factor 2.

Biosynthesis begins withtransfer of the 3-amino-3-carboxypropyl (ACP) group of S-adenosylmethionine(AdoMet)to the imidazole C-2 of the precursor histidine residue. Trimethylation of newlyformed amino group using AdoMet as a source of methyl group leads to theformation of Diphthine. Diphthine is converted to Diphthamide by ATP dependentamidation of carboxyl group. Mutants defective in Diphthamide biosynthesis havebeen isolated by selection for resistance to the actions of Diphtheria toxinand exotoxin A.These mutations are categorized into five complementation groups     

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