Cell also be applied in the analysis

Cell and Molecular Biology uses different techniques and methods in dealing with the structural unit of the living system. Several laboratory instruments are used to attain accuracy and precision.
Micropipettes are commonly used instruments in attaining precision. It is a Winconsin invention which was developed by both Warren Gilson, an inventor and Henry Lardy, a biochemistry professor. Three different pipettes are used for accurate measuring and dispensing small liquid volumes. A small volume micropipette has a range of 0.5-10 ?L, a mid range micropipette has a volume capacity of 10-100?L and a large volume micropipette which can handle 100-1000?L.
A spectrophotometer is used to determine how much light was absorbed in a certain chemical substance by measuring the intensity of light as it passes through the solution. It can also be applied in the analysis of a purity of a substance qualitatively, quantitation of DNA and protein, measurements of cell analysis and assays involving enzyme-catalyzed reactions. The UV-VIS spectrophotometer is used to qualitatively determine solutions with low concentrations. It compares the intensity of the transmitted light with the incident light.
Beer-Lambert Law is used to compute for the expected absorbance. It consists of two laws: Lambert’s Law, which is concerned with the thickness of the medium or cuvette and the Beer’s Law which is concerned with the concentration of the solution. It uses the equation A=?cL whereas A, is the absorbance. ?, is the molar extinction coefficient having the value (1.98×104 L)/ mol.cm and L, the length of sample in its standard 1cm.


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