Cell Respiration Lab ReportGriffin Yates, Ben Fandey, Sofia Musgrove, and Christina TangSage Creek High SchoolJanuary 19, 2018AbstractThe purpose for us doing a lab report is to give a keen, informational description on the Lab we were told to do. To complete the lab report we decided each of us should do one to two parts of it while following and looking up info for each individuals part. As we look at the results of the lab we found that the increase in CO2 accord at a faster rate when the students ran stairs. The conclusion for this experiment can be proven through the scientific theories about cell respirations as the more oxygen is introduced the more carbon dioxide is produced. Background Cellular respiration is the process used to turn glucose into energy (ATP). The reactants are glucose and oxygen and the products are carbon dioxide, water, and ATP.
The equation for cellular respiration is: C6H12O6, + 602 6CO2 + 6H2O + ATP. There are two types of cellular respiration: aerobic and anaerobic. In aerobic cellular respiration, oxygen is required to break down glucose and creates 36 ATP molecules. There are three steps to the aerobic cellular respiration: glycolysis, the Krebs Cycle, and the electron transport chain (ETC). Aerobic cellular respiration takes place in the inner mitochondrial membrane, specifically in the Cristae. In glycolysis, glucose is broken down into 2 molecules of pyruvate (Khan Academy, 2016). In the Krebs Cycle, pyruvate diffuses into the inner matrix of the mitochondria and is converted into carbon dioxide and acetyl.
2 ATP, 6 NADH, and 2 FADH are created in this step. In the ETC, electrons are released from NADH and FADH and provide energy to make ATP. Oxygen also acts as the final electron acceptor that drives this process and makes water. On the other hand, anaerobic cellular respiration the process of producing cellular energy without oxygen (Roisen, 2017).
Only glycolysis occurs and 2 ATPs are produced. Respiration was measured by breathing through a straw into bromothymol blue solution (BTB) and measuring the time it took the water to change from blue to yellow (Schoolworkhelper, 2016). Bromothymol blue changes color from yellow (6) to blue (7.
6) over a pH range because it is a pH indicator. Factors like temperature, light, etc. may influence the rate of respiration (Kumar, 2015). For example in rice, oxygen is removed and the rate of respiration increases according to the carbon dioxide created.
In this lab, different activities were used to test how they affected the rate of cellular respiration. A stopwatch and bromothymol solution were used to record the rate of respiration. The types of activities that were carried out affected the rate of respiration. When a student held their breath or sprinted on the stairs, there was a noticeable decrease compared to our control group. This lab was designed to answer the question of, “How do different activities affect the rate of cellular respiration?” Different activities allowed students to see the impact that exercise had on cellular respiration. Hypothesis When someone holds their breath in trial two, there should be a faster rate of respiration.When someone is sprinting up and down stairs in trial three, there should be a faster rate of respiration. The independent variable is the amount of activity someone is doing.
The dependant variable is the measure of time the color should change which is the rate of the CO2.Materials and Procedures Materials needed: -a stopwatch- graduated cylinder-small beaker-bromothymol blue solution (BTB)-three to four straws-safety gogglesProcedures:First, a graduated cylinder was used to measure twenty milliliters of tap water to pour it into a small beaker. After that, 8 drops of bromothymol blue were added with a dropper into the water to create a bromothymol blue solution. Then, carbon dioxide was carefully exhaled into the BTB solution with a straw. As the student was exhaling, they were timed for how long it took for the BTB solution to go from blue to yellow and recorded it. Finally to avoid contamination, the beaker gets emptied, cleaned, and dried.
Repeat steps 1-5 with each student and for every trial. ResultsStudent 1Student 2Student 3Student 4AverageTrial 1:11 secs31 secs21 secs20 secs21 secsTrial 2:7 secs9 secs10 secs12 secs9 secsTrial 3:9 secs9 secs8 secs7 secs8.25 secsEquation: C6H12O6, + 602 6CO2 + 6H2O + ATP From the results, a conclusion can be made that trial 2 had the fastest rate of carbon dioxide produce as the students were told to run stairs until they were breathing heavily. This is seen in the data as the average for trial 3 was 8.28 specs compared to trial 2’s 9 secs and trial 1’s 21 secs.DiscussionThe hypothesis for trial two was that if someone holds their breath, then there should be a faster rate of respiration. This hypothesis was supported because as someone held their breath, the time that the BTB solution took an average of 9 seconds to change from blue to yellow.
These results make sense because as the time decreased, it indicated that the rate of respiration increased. The hypothesis for trial three was that if someone sprinted up and down on stairs, then there should be a faster rate of respiration. This hypothesis was supported because as someone sprinted up and down the stairs, the time that the BTB solution took an average of 8.25 seconds to change from blue to yellow. These results make sense because as the time decreased, it indicated that the rate of respiration increased. Numerous errors occurred throughout the lab. Not all students exhaled at the same speed giving the students results that are not as accurate.
There should have been a beaker of already yellow BTB solution to compare the one being exhaled into. Every student also should have held their breath sprinted on the stairs for the same amount of times. This lab is important because students get to see how exercise affects their rate of cellular respiration. When individuals exercise, their body intakes more oxygen producing more carbon dioxide therefore increasing their heart rate and cellular respiration. ConclusionThe experiment tested and proved that breathing, using more energy, has a direct effect into the BTB solution changing colors because there was more oxygen being used up by the body, releasing more carbon dioxide when exhaled.
Citations Schoolworkhelper Editorial Team. (2016). Bromothymol Blue & Aerobic Respiration. Retrieved January 20, 2018, from https://schoolworkhelper.net/bromothymol-blue-aerobic-respiration/Kumar, S.
(2015, October 26). Factors Affecting Aerobic Respiration: 8 Factors | Plants. Retrieved January 20, 2018, from http://www.biologydiscussion.com/respiration/aerobic-respiration/factors-affecting-aerobic-respiration-8-factors-plants/15206Roisen, P.
(2017). Anaerobic Respiration.Retrieved January 20, 2018, from https://www.brightstorm.com/science/biology/cell-functions-and-processes/anaerobic-respiration/(2016). Steps of cellular respiration. Retrieved January 20, 2018, from https://www.khanacademy.org/science/biology/cellular-respiration-and-fermentation/overview-of-cellular-respiration-steps/a/steps-of-cellular-respiration