Biochemical techniques and this analytical technique is known

Biochemical techniques are used basically for theidentification and separation of the particular compounds Following are the biochemical techniques ·       Chromatography ·       Electrophoresis·       Centrifugation·       Mass Spectroscopy·       Spectrometry·       Nuclear Magnetic Resonance (NMR)·       Photometry·       Atomic Absorbance Spectroscopy·       Electron spin Spectroscopy CHROMATOGRAPHYA technique which is used in the separation of mixture isknown as chromatographic technique. Chromatography technique is a laboratorytechnique.

SIGNIFICANCEThe importance of the chromatography is versatility andsmall quantities of a material can be separated with case it is fast andaccurate               MASSSPECTROSCOPYIt is analytical techniques in which chemical species areionized and these ions are sorted based on their mass to charge ratio  SIGNIFICANCEMass spectroscopy is used in pharmaceutical, clinical,environmental, Biotechnology                   SPECTROPHOTOMETRYSpectrophotometry is commonly used for the measurement oftransmittance of reflectance of solution, transparent or opaqueSIGNIFICANCEIts importance in medical research, development ofmedicine research on vitamin and hormones etc.                  ATOMICABSORPTION SPECTROSCOPYConcentration of the elements is measured by the analyticaltechniques and this analytical technique is known as atomic absorptionspectroscopyIt is so sensitive that it can measure down to part perbillion of gram per sample SIGNIFICANCEIt is applied in clinical analysis, environmentalanalysis and pharmaceutical industries           ELECTRONSPIN SPECTROSCOPYIt can measure the transition frequency between differentelectron pin statesEMR is used to study molecules or atoms with an unpairedelectron SIGNIFICANCEIt is widely used in research of biological free radicalsfor qualitative and quantitative analysis of reactive O2 radicals and reactivenitrogen radicals     NUCLEARMAGNETIC RESONANCEAt certain atomic level the magnetic properties are exploitingby NMR SIGNIFICANCEImportance in to investigate the properties of organicmolecules by chemist and bio chemist CENTRIFUGATIONCentrifugation is a process in which the centrifugalforce are applied for the sedimentation of the heterogeneous mixture with acentrifugeSIGNIFICANCEUsed for the separation of molecules on the basis ofdensity and used in industries research ELECTROPHORESISEIt is the motion of dispersed particle relative to afluid under the influence of spatially uniform electric fieldSIGNIFICANCEImportance in the separation of macromolecules such DNA,RNA and protein

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