Bacterial Structures Lab Endospores

Bacterial Structures Lab Endospores, Flagellum, Slime Layers Purpose To observe some structures of bacteria To understand the anatomy of bacterial endospores, flagellum, capsules and slime layers To understand the physiology of bacterial endospores, flagellum, capsules and slime layers To practice and develop microscope skills Materials Microscope Immersion Oil Lens paper Lens cleaner Prepared slide of Bacterial Spores Prepared slide of Bacterial Capsules Prepared slide of Bacterial Flagella Part A Obtain a prepared slide labeled Bacterial Spores reference pictures found in your textbook on pg. 98 99. Begin with the shortest object (4x) and the stage in the highest position. Carefully focus the slide to a clear image using the coarse adjustment knob Center the image of Rotate the 10x objective into place and again focus the slide using the fine adjustment knob Rotate the 40x objective into place and again focus the slide using the fine adjustment knob. Rotate you objective so that the 40x objective is on one side of hole in the stage and the 100x objective in on the other side Place one drop of immersion oil on the microscope slide where the hole is visible. Swing the 100x objective into place and focus on using the fine adjustment knob. Bacterial Endospore questions Name the three genera of bacteria that produce endospores Name five environmental conditions or stresses endospores are resistant to. How long can bacteria with endospores remain viable Name three endospore forming bacteria that cause disease in humans. Can endospores survive in boiling water Can endospores be seen with the light microscope Describe the germination of endospores. What is a vegetative cell. Part B Obtain a prepared slide labeled Bacterial Capsules reference pictures found in your textbook on pg. 91. Begin with the shortest object (4x) and the stage in the highest position. Carefully focus the slide to a clear image using the coarse adjustment knob Center the image of Rotate the 10x objective into place and again focus the slide using the fine adjustment knob Rotate the 40x objective into place and again focus the slide using the fine adjustment knob. Rotate you objective so that the 40x objective is on one side of hole in the stage and the 100x objective in on the other side Place one drop of immersion oil on the microscope slide where the hole is visible. Swing the 100x objective into place and focus on using the fine adjustment knob. Capsules, Slime Layers and S-layer questions What is the difference between a capsule and a slime layer What are capsules and slime layers usually composed of Which is easier to observe with a light microscope a capsule or a slime layer Name three advantages of a capsule. Name one advantage of a slime layer. What is a S-layer usually composed of Name three advantages of a S-layer. Name a bacterium with an S-layer. Part C Obtain a prepared slide labeled Bacterial Flagella reference pictures found in your textbook on pg. 87. Begin with the shortest object (4x) and the stage in the highest position. Carefully focus the slide to a clear image using the coarse adjustment knob Center the image of Rotate the 10x objective into place and again focus the slide using the fine adjustment knob Rotate the 40x objective into place and again focus the slide using the fine adjustment knob. Rotate you objective so that the 40x objective is on one side of hole in the stage and the 100x objective in on the other side Place one drop of immersion oil on the microscope slide where the hole is visible. Swing the 100x objective into place and focus on using the fine adjustment knob. Flagella and Motility questions Name the two major groups that appendages can be divided into. Why must your use high magnification to see flagella under a light microscope Identify the three parts of a bacterial flagellum. How can motility be assessed in bacteria. Explain a run. Explain tumbles. What inhibits tumbles What is phototaxis Y, B8L 1(IzZYrH9pd4n(KgVB,lDAeX)Ly5otebW3gpj/gQjZTae9i5j5fE514g7vnO( ,[email protected] /[email protected] 6Q